Featured Model: Cetnar, D. P., & Salis, H. M. (2021). Systematic Quantification of Sequence and Structural Determinants Controlling mRNA stability in Bacterial Operons. ACS Synthetic Biology, 10(2), 318-332.

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Simultaneous Regulation of Many Genes using Extra Long sgRNA Arrays

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Articles to Cite

Reis A.C, Halper S.M., Vezeau G.E., Cetnar D.P., Hossain A., Clauer P.R., and Salis H.M. Simultaneous repression of multiple bacterial genes using highly non-repetitive extra-long sgRNA arrays. Nature Biotechnology. v37(11), p1294-1301 (2019).
API Power Mode Documentation.

#!/usr/bin/python import requests, json, hmac, hashlib, datetime #Create an input parameter dictionary params['title'] = '' params['numTargets'] = '10' params['guide_list'] = '{"0":{"sequence":""},"1":{"sequence":""},"2":{"sequence":""},"3":{"sequence":""},"4":{"sequence":""},"5":{"sequence":""},"6":{"sequence":""},"7":{"sequence":""},"8":{"sequence":""},"9":{"sequence":""},"10":{"sequence":""},"11":{"sequence":""},"12":{"sequence":""},"13":{"sequence":""},"14":{"sequence":""},"15":{"sequence":""},"16":{"sequence":""},"17":{"sequence":""},"18":{"sequence":""},"19":{"sequence":""}}' params['target_list'] = '{"0":{"sequence":"","sgRNAsPerTarget":""},"1":{"sequence":"","sgRNAsPerTarget":""},"2":{"sequence":"","sgRNAsPerTarget":""},"3":{"sequence":"","sgRNAsPerTarget":""},"4":{"sequence":"","sgRNAsPerTarget":""},"5":{"sequence":"","sgRNAsPerTarget":""},"6":{"sequence":"","sgRNAsPerTarget":""},"7":{"sequence":"","sgRNAsPerTarget":""},"8":{"sequence":"","sgRNAsPerTarget":""},"9":{"sequence":"","sgRNAsPerTarget":""},"10":{"sequence":"","sgRNAsPerTarget":""},"11":{"sequence":"","sgRNAsPerTarget":""},"12":{"sequence":"","sgRNAsPerTarget":""},"13":{"sequence":"","sgRNAsPerTarget":""},"14":{"sequence":"","sgRNAsPerTarget":""},"15":{"sequence":"","sgRNAsPerTarget":""},"16":{"sequence":"","sgRNAsPerTarget":""},"17":{"sequence":"","sgRNAsPerTarget":""},"18":{"sequence":"","sgRNAsPerTarget":""},"19":{"sequence":"","sgRNAsPerTarget":""}}' params['organism_type'] = prokaryotic params['algorithm'] = 'ELSACalculator_DesignMode' params['algorithm_version'] = 'v1.0' #Send it to https://api.denovodna.com/ using a HTTPS POST params['publicConsumerToken'] = 'test' params['timestamp'] = datetime.datetime.utcnow().isoformat() params['signature'] = hmac.new('private key', message, hashlib.sha1).hexdigest() message = publicConsumerToken + params['timestamp'] + 'undefined' headers = {'Content-type': u'application/json', 'Accept': u'application/json'} r = requests.post('https://api.denovodna.com/ELSACalculator_DesignMode', data=json.dumps(params), headers=headers) output = r.json() #Retrieve the result after the calculation has completed. id = output['JobInfo']['id'] result = sendGet('Result',{'id' : id})