The Salis Lab has recently discovered a new mechanism, called Ribosome Drafting, that controls a mRNA's translation rate. Learn more about it in our latest publication: Espah Borujeni and Salis. Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism, Journal of the American Chemical Society, 2016

Are you engineering riboswitches? See our latest NAR Breakthrough Article Automated physics-based design of riboswitches from diverse RNA aptamers, Nucleic Acid Research, 2016. Are you engineering bacterial operons? In non-model hosts? Then our latest published work should also interest you: Kushwaha and Salis, A Portable Expression Resource for Engineering Cross-species Genetic Circuits and Pathways, Nature Communications, 2015. and Tian and Salis, A Predictive Biophysical Model of Translational Coupling to Coordinate and Control Protein Expression in Bacterial Operons, Nucleic Acid Research, 2015


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Protein Coding Sequence #1
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RBS Sequence[?]
Ribosome Binding Site Sequence: enter the nucleotide sequence that controls a mRNA's translation initiation rate, using A/G/C/T/U. A complete RBS sequence is 35 nucleotides long.
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Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U.
Begins with a start codon (ATG/GTG/TTG/CTG). (required)
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Protein Coding Sequence #2
CDS Name:
RBS Sequence[?]
Ribosome Binding Site Sequence: enter the nucleotide sequence that controls a mRNA's translation initiation rate, using A/G/C/T/U. A complete RBS sequence is 35 nucleotides long.
:

CDS Sequence (nt only) [?]
Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U.
Begins with a start codon (ATG/GTG/TTG/CTG). (required)
:

Protein Coding Sequence #3
CDS Name:
RBS Sequence[?]
Ribosome Binding Site Sequence: enter the nucleotide sequence that controls a mRNA's translation initiation rate, using A/G/C/T/U. A complete RBS sequence is 35 nucleotides long.
:

CDS Sequence (nt only) [?]
Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U.
Begins with a start codon (ATG/GTG/TTG/CTG). (required)
:
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We gratefully acknowledge research funding from the Air Force Office of Scientific Research, the National Science Foundation, the Office of Naval Research, and an Amazon AWS Research Grant.
Computational resources are provided by the AWS Elastic Compute Cloud.